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The pH of the mixture was changed to acidic by three rounds of concentrating and diluting with 0.1 M sodium acetate, 300 mM ammonium sulfate (pH 4.2) buffer using 100-kDa Amicon ultra centrifugal filters.

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Corse Project Guide - Free download as Word Doc (.doc / .docx), PDF File (.pdf), Text File (.txt) or read online for free. Course Project Guide on Machine Elements Design Of Single Stage Speed Reducer

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